CMPK2 Polyclonal antibody

CMPK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25877-1-AP

Synonyms

UMP-CMP kinase 2, mitochondrial, UMP CMPK2, TYKi, Nucleoside-diphosphate kinase, EC:2.7.4.6

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Validation Data Gallery

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  WB analysis using 25877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25877-1-AP (CMPK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 25877-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25877-1-AP (CMPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

25877-1-AP targets CMPK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CMPK2 fusion protein Ag22885
• Full Name: cytidine monophosphate (UMP-CMP) kinase 2, mitochondrial
• Calculated Molecular Weight: 449 aa, 49 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC141802
• Gene Symbol: CMPK2
• Gene ID (NCBI): 129607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CMPK2 belongs to the thymidylate kinase family. CMPK2 may participate in dUTP and dCTP synthesis in mitochondria. It is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy of CMPK2 is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. CMPK2 may be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs.

Publications

Species	Application	Title
human	IHC	Front Genet; Mitochondrial-related hub genes in dermatomyositis: muscle and skin datasets-based identification and in vivo validation; Authors - Shuo Wang
human	WB	Sci Rep; LincRNA-p21/AIF-1/CMPK2/NLRP3 pathway promoted inflammation, autophagy and apoptosis of human tubular epithelial cell induced by urate via exosomes; Authors - Jianbing Hao
human	WB	Cell Biochem Biophys; CMPK2 Promotes CD4+ T Cell Activation and Apoptosis through Modulation of Mitochondrial Dysfunction in Systemic Lupus Erythematosus; Authors - Ya-Nan Tan ; KD Validated
mouse	WB	Int J Biol Sci; The FTO-CMPK2 Pathway in Fibroblast-like Synoviocytes Modulates Rheumatoid Arthritis Synovial Inflammation and Cartilage Homeostasis via mtDNA Regulation; Authors - Li Jin