CMIP Recombinant antibody

CMIP Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240059C12

Cat no : 83381-1-RR

Synonyms

Tc Mip, c-Mip, C-Maf-inducing protein, c Mip, c Maf inducing protein

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Validation Data Gallery

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  WB analysis of human peripheral blood platelets using 83381-1-RR

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 83381-1-RR (CMIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83381-1-RR (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83381-1-RR (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A549 using 83381-1-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug CMIP Recombinant Antibody (83381-1-RR, Clone:240059C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: human peripheral blood platelets
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83381-1-RR is CMIP Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CMIP fusion protein Ag3475
• Full Name: c-Maf-inducing protein
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC038113
• Gene Symbol: CMIP
• Gene ID (NCBI): 80790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene of CMIP was isolated from one patient of minimal change nephrotic syndrome. The gene encodes two isoforms: CMIP and truncated CMIP. The predicted CMIP and truncated CMIP proteins contain 739 and 679 amino acids and have calculated molecular masses of 85 and 75 kD, respectively. The two isoforms have different distribution and expression pattern.