CLUAP1 Recombinant antibody

CLUAP1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240854C10

Cat no : 83849-3-RR

Synonyms

Qilin, KIAA0643, Clusterin-associated protein 1, clusterin associated protein 1, 240854C10

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Validation Data Gallery

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  WB analysis using 83849-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83849-3-RR (CLUAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83849-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83849-3-RR against Human CLUAP1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: Y79 cells, HepG2 cells, COLO 320 cells, HeLa cells, BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83849-3-RR is CLUAP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLUAP1 fusion protein Ag11419
• Full Name: clusterin associated protein 1
• Calculated Molecular Weight: 413 aa, 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC017070
• Gene Symbol: CLUAP1
• Gene ID (NCBI): 23059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in the testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261).