Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 12289-1-AP


AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver tissue, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12289-1-AP targets Clusterin in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Clusterin fusion protein Ag2889
Full Name clusterin
Calculated Molecular Weight 449 aa, 53 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).


Product Specific Protocols
WB protocol for Clusterin antibody 12289-1-APDownload protocol
IHC protocol for Clusterin antibody 12289-1-APDownload protocol
IF protocol for Clusterin antibody 12289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Proc Natl Acad Sci U S A

Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.

Authors - Lei Shi

Sci Signal

Platelets contribute to amyloid-β aggregation in cerebral vessels through integrin αIIbβ3-induced outside-in signaling and clusterin release.

Authors - Lili Donner

Front Cell Dev Biol

Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes.

Authors - Xinlei Li

Ecotoxicol Environ Saf

Identification and functional analysis of senescence-associated secretory phenotype of premature senescent hepatocytes induced by hexavalent chromium.

Authors - Yu Ma

Food Chem Toxicol

Clusterin overexpression as a potential neuroprotective response to the pathological effects of high fat dieting on the brain reward system.

Authors - Carmen Rodríguez-Rivera

Ecotoxicol Environ Saf

Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.

Authors - Yujing Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


George (Verified Customer) (12-03-2018)

IHC performed on paraffin-embedded human kidney tissue

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: human
Clusterin Antibody Immunohistochemistry, validation (1:200 dilution) in human (Cat no:12289-1-AP)