Filter: AllWBIHCIF/ICC

WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)
WB result of CLTC antibody (66487-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLTC transfected HeLa cells. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)
HeLa, Jurkat, ROS1728 cells, and pig brain tissue were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 66487-1-Ig (same clone as 66487-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66487-1-Ig (same clone as 66487-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66487-1-Ig (same clone as 66487-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66487-1-Ig (same clone as 66487-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CLTC antibody (66487-1-Ig, Clone: 1B3D7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66487-1-Ig (CLTC antibody), at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CLTC Monoclonal antibody, PBS Only

CLTC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66487-1-PBS

Clone No.1B3D7

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17, CHC

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66487-1-PBS targets CLTC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity	human, mouse, rat, pig, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CLTC fusion protein Ag25040 Predict reactive species
Full Name	clathrin, heavy chain (Hc)
Calculated Molecular Weight	192 kDa
Observed Molecular Weight	170 kDa
GenBank Accession Number	BC054489
Gene Symbol	CLTC
Gene ID (NCBI)	1213
RRID	AB_2881852
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q00610
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)