CLSTN2 Polyclonal antibody

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  WB analysis using 21436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21436-1-AP (CLSTN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21436-1-AP (CLSTN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CLSTN2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21436-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Calsyntenin-2, calsyntenin 2, Alc-gamma, Alcadein-gamma, Alcadein gamma

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

21436-1-AP targets CLSTN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLSTN2 fusion protein Ag16048
• Full Name: calsyntenin 2
• Calculated Molecular Weight: 955 aa, 107 kDa
• Observed Molecular Weight: 100-107 kDa
• GenBank Accession Number: BC104485
• Gene Symbol: CLSTN2
• Gene ID (NCBI): 64084
• RRID: AB_3085656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4D0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLSTN2 gene encodes the synaptic protein calsyntenin 2, one of the three calsyntenin family members found in humans. CLSTN2 is expressed exclusively in the brain, with high levels occurring in cortical gamma-aminobutyric acid (GABA) interneurons and in medial temporal lobe regions (PMID: 19058786). Western blot analysis detected CLSTN2 at an apparent molecular mass of 107 kDa.

Publications

Species	Application	Title
	IHC	Clin Transl Med; EHMT2-mediated R-loop formation promotes the malignant progression of prostate cancer via activating Aurora B; Authors - Yuyang Zhang