Claspin Polyclonal antibody

Claspin Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 23206-1-AP

Synonyms

hClaspin, CLSPN

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Validation Data Gallery

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  WB analysis of HEK-293 using 23206-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23206-1-AP (Claspin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 23206-1-AP

  IP result of anti-Claspin (IP:23206-1-AP, 5ug; Detection:23206-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

• 

  IHC staining of human stomach cancer using 23206-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23206-1-AP (Claspin antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 23206-1-AP

  Immunofluorescent analysis of HepG2 cells using 23206-1-AP (Claspin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HeLa using 23206-1-AP

  Immunofluorescent analysis of HeLa cells using 23206-1-AP (Claspin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

23206-1-AP targets Claspin in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claspin fusion protein Ag19637
• Full Name: claspin homolog (Xenopus laevis)
• Calculated Molecular Weight: 1339 aa, 151 kDa
• Observed Molecular Weight: 240 kDa
• GenBank Accession Number: BC137279
• Gene Symbol: Claspin
• Gene ID (NCBI): 63967
• RRID: AB_2879232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLSPN, also named as Claspin, is a 1339 amino acid protein, which belongs to the claspin family. CLSPN localizes in the nucleus and is highly expressed at S/G2 phases of the cell cycle. CLSPN is required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. CLSPN plays a potentially critical role in replication checkpoint control in cells and mediating phosphorylation and activation of checkpoint protein kinases. The calculated molecular weight of CLSPN is 151 kDa, but the modified protein is about 220-240 kDa.

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.; Authors - Uma Thanigai Arasu
human	WB	Int J Oncol; Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells.; Authors - Chao Wang
human	WB	Cell Insight; ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance; Authors - Rong Luo