WB result of CLPX antibody (31050-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLPX transfected A549 cells.
WB analysis using 31050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31050-1-AP (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 31050-1-AP (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of mouse stomach using 31050-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31050-1-AP (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
31050-1-AP targets CLPX in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
WB result of CLPX antibody (31050-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLPX transfected A549 cells.
WB analysis using 31050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31050-1-AP (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse stomach using 31050-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31050-1-AP (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
CLPX Polyclonal antibody
Catalog Number
31050-1-AP
Citations
-
Dilutions
WB : 1:2000-1:10000 IHC : 1:50-1:500
Applications
WB, IHC, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
