CLPTM1 Polyclonal antibody

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  WB analysis using 15337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15337-1-AP (CLPTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 15337-1-AP

  IP result of anti-CLPTM1 (IP:15337-1-AP, 4ug; Detection:15337-1-AP 1:300) with A549 cells lysate 1050 ug.

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  IHC staining of mouse brain using 15337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15337-1-AP (CLPTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15337-1-AP (CLPTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CLPTM1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15337-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Putative lipid scramblase CLPTM1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15337-1-AP targets CLPTM1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLPTM1 fusion protein Ag7538
• Full Name: cleft lip and palate associated transmembrane protein 1
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: BC004865
• Gene Symbol: CLPTM1
• Gene ID (NCBI): 1209
• RRID: AB_3669204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O96005
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleft lip and palate transmembrane protein 1 (CLPTM1), a multipass transmembrane protein, was identified due to the association of a chromosomal translocation with a cleft lip and palate (PMID: 9828125). CLPTM1 regulates epileptic seizures by modulating GABAAR-mediated inhibitory synaptic transmission (PMID: 36519412). Highly expressed CLPTM1L may contribute to a poor prognosis and increase invasion, proliferation and migration of oral squamous cell carcinoma (PMID: 34586883).