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  • KD/KO Validated

CLPP Monoclonal antibody

CLPP Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E1D9

Cat no : 66271-1-Ig

Synonyms

CLPP, Endopeptidase Clp



Tested Applications

Positive WB detected inhuman heart tissue, pig heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66271-1-Ig targets CLPP in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen CLPP fusion protein Ag8420
Full Name ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
Calculated molecular weight 30 kDa
Observed molecular weight 26 kDa, 30 kDa
GenBank accession numberBC002956
Gene symbol CLPP
Gene ID (NCBI) 8192
RRIDAB_2881656
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Protocols

Product Specific Protocols
WB protocol for CLPP antibody 66271-1-IgDownload protocol
IHC protocol for CLPP antibody 66271-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Enhancing mitochondrial proteostasis reduces amyloid-β proteotoxicity.

Authors - Vincenzo Sorrentino
humanIF

Cells

Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.

Authors - Jana Key
mouseWB

PeerJ

Mitochondrial unfolded protein response gene CLPP changes mitochondrial dynamics and affects mitochondrial function.

Authors - GuiJun Wu
  • KD Validated
humanWB

NeuroImmune Pharm Ther

Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages

Authors - Santosh Kumar