CLPB Polyclonal antibody

CLPB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15743-1-AP

Synonyms

SKD3, Mitochondrial disaggregase, cleaved form, Mitochondrial disaggregase, HSP78, EC:3.6.1.-

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Validation Data Gallery

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  WB analysis using 15743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15743-1-AP (CLPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15743-1-AP (CLPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 15743-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15743-1-AP (CLPB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 15743-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15743-1-AP (CLPB Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 15743-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CLPB antibody (15743-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

15743-1-AP targets CLPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLPB fusion protein Ag8165
• Full Name: ClpB caseinolytic peptidase B homolog (E. coli)
• Calculated Molecular Weight: 707 aa, 79 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC006404
• Gene Symbol: CLPB
• Gene ID (NCBI): 81570
• RRID: AB_2847900
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLPB encodes a protein of 707 aa with a predicted molecular mass of approximately 79 kDa, presenting two conserved ATP-binding domains. CLPB is a member of the AAA+ protein family. This potential internal translation initiation site suggests that a smaller form of ClpB, with a theoretical molecular mass of approximately 58 kDa, may be produced. (PMID: 25597510, PMID: 255957260)

Publications

Species	Application	Title
	WB	Sci Adv; Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding; Authors - Arpit Gupta
human	WB	Elife; Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations.; Authors - Ryan R Cupo
human	WB	iScience; Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region.; Authors - Takahiro Yoshinaka
mouse	WB	Elife; Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis; Authors - Enrico Radaelli