Tested Applications
Positive WB detected in | HEK-293 cells, NIH/3T3 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
24605-1-AP targets CLN5 in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CLN5 fusion protein Ag18175 Predict reactive species |
Full Name | ceroid-lipofuscinosis, neuronal 5 |
Calculated Molecular Weight | 407 aa, 46 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | BC153154 |
Gene Symbol | CLN5 |
Gene ID (NCBI) | 1203 |
RRID | AB_3662025 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O75503 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The CLN5 (ceroid lipofuscinosis neuronal protein 5) protein is ubiquitously expressed in the majority of tissues studied and in the brain, CLN5 shows both neuronal and glial cell expression. CLN5 plays a role in maintaining an acidic environment in the lysosomes, a critical feature for a functional lysosome (PMID: 38236309). Moreover, CLN5-deficient neuronal progenitor cells showed reduced thioesterase activity, confirming the live cell function of CLN5 in setting S-depalmitoylation levels (PMID: 35427157).
Protocols
Product Specific Protocols | |
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WB protocol for CLN5 antibody 24605-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |