CLIP4 Polyclonal antibody

CLIP4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17044-1-AP

Synonyms

RSNL2, Restin-like protein 2, CAP-Gly domain-containing linker protein 4

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Validation Data Gallery

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  WB analysis of Y79 using 17044-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17044-1-AP (CLIP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 17044-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 17044-1-AP (CLIP4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HUVEC using 17044-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 17044-1-AP (CLIP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HUVEC cells, Y79 cells
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17044-1-AP targets CLIP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLIP4 fusion protein Ag10590
• Full Name: CAP-GLY domain containing linker protein family, member 4
• Calculated Molecular Weight: 705 aa, 76 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: BC015310
• Gene Symbol: CLIP4
• Gene ID (NCBI): 79745
• RRID: AB_2878341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Signal; miR-135b-5p promotes gastric carcinogenesis by targeting CLIP4-mediated JAK2/STAT3 signal pathway; Authors - Zhiwei Peng