CLIP-115/CLIP2 Monoclonal antibody

• Featured Product
• KD/KO Validated

CLIP-115/CLIP2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6B6

Cat no : 67776-1-Ig

Synonyms

CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 67776-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67776-1-Ig

  WB result of CLIP-115/CLIP2 antibody (67776-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

• 

  WB analysis of pig cerebellum using 67776-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 67776-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 67776-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67776-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67776-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67776-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 67776-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CLIP-115/CLIP2 antibody (67776-1-Ig, Clone: 2E6B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, rabbit brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, A549 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67776-1-Ig targets CLIP-115/CLIP2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CLIP-115/CLIP2 fusion protein Ag18902
• Full Name: CAP-GLY domain containing linker protein 2
• Calculated Molecular Weight: 1011 aa, 112 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC150332
• Gene Symbol: CLIP2
• Gene ID (NCBI): 7461
• RRID: AB_2918541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome.