CLEC12B Polyclonal antibody

CLEC12B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26077-1-AP

Synonyms

CLEC12B, Macrophage antigen H, UNQ5782

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Validation Data Gallery

• 

  WB analysis of mouse brain using 26077-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26077-1-AP (CLEC12B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 26077-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26077-1-AP (CLEC12B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26077-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26077-1-AP (CLEC12B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 26077-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26077-1-AP (CLEC12B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, K-562 cells, mouse spleen tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26077-1-AP is CLEC12B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLEC12B fusion protein Ag23396
• Full Name: C-type lectin domain family 12, member B
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC136805
• Gene Symbol: CLEC12B
• Gene ID (NCBI): 387837
• RRID: AB_2880365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC12B (C-type lectin domain family 12 member B) is a single-pass type II membrane protein of the C-type lectin-like family. CLEC12B acts as an inhibitory receptor on myeloid cells. CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. (PMID: 17562706)