CLEC12A Polyclonal antibody

CLEC12A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28627-1-AP

Synonyms

C-type lectin domain family 12 member A, CLL-1, CLL1, CLL 1, CD371

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Validation Data Gallery

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  WB analysis of U-937 using 28627-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 28627-1-AP (CLEC12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28627-1-AP

  Human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 28627-1-AP (CLEC12A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of U-937 using 28627-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-937 cells without permeabilization using CLEC12A antibody (28627-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: U-937 cells, human peripheral blood leukocyte
• Positive IF/ICC detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28627-1-AP targets CLEC12A in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLEC12A fusion protein Ag27864
• Full Name: C-type lectin domain family 12, member A
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 55-75 kDa
• GenBank Accession Number: BC063424
• Gene Symbol: CLEC12A
• Gene ID (NCBI): 160364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).