Claudin 7 Polyclonal antibody

Claudin 7 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29795-1-AP

Synonyms

CEPTRL2, Claudin 7, CLDN 7, CLDN7, CPETRL2, Hs.84359

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Validation Data Gallery

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  IHC staining of human oesophagus cancer using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human colon tissue, mouse colon tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

29795-1-AP targets Claudin 7 in IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 7 fusion protein Ag31706
• Full Name: claudin 7
• Calculated Molecular Weight: 22.4 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC001055
• Gene Symbol: Claudin 7
• Gene ID (NCBI): 1366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publications

Species	Application	Title
mouse	IF	J Agric Food Chem; Lacticaseibacillus rhamnosus KY16 Improves Depression by Promoting Intestinal Secretion of 5-HTP and Altering the Gut Microbiota; Authors - Shicai Xie