CLCN7 Polyclonal antibody

CLCN7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29230-1-AP

Synonyms

OPTA2, H(+)/Cl(-) exchange transporter 7, ClC-7, CLC7, CLC 7

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Validation Data Gallery

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  WB analysis using 29230-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29230-1-AP (CLCN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue, mouse small intestine mouse small intestine, rat small intestine rat small intestine

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29230-1-AP targets CLCN7 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLCN7 fusion protein Ag29882
• Full Name: chloride channel 7
• Calculated Molecular Weight: 805 aa, 89 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC012737
• Gene Symbol: CLCN7
• Gene ID (NCBI): 1186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The intracellular CLC chloride channel family members ClC-3, ClC-4, and ClC-5 have been proposed as the primary endosomal chloride conductance providers. ClC-7 is expressed in late endosomes and lysosomes, but there is disagreement in the literature regarding its contribution to acidification. More recently, the cystic fibrosis (CF) transmembrane conductance regulator chloride channel (CFTR) was proposed as the specific counter-ion conductance necessary for lysosomal acidification in alveolar macrophages. (PMID: 20566682)