CLCA2 Polyclonal antibody

CLCA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19273-1-AP

Synonyms

CACC, CaCC 3, CACC3, chloride channel accessory 2, CLCA2, CLCRG2, hCaCC 3, hCLCA2

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Validation Data Gallery

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  WB analysis of mouse lung using 19273-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19273-1-AP (CLCA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19273-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19273-1-AP (CLCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 19273-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 19273-1-AP (CLCA2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 19273-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 19273-1-AP (CLCA2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue, COLO 320 cells
• Positive IHC detected in: human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 2 publications below

Product Information

The immunogen of 19273-1-AP is CLCA2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLCA2 fusion protein Ag5914
• Full Name: chloride channel accessory 2
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 141 kDa, 35-40 kDa
• GenBank Accession Number: BC041096
• Gene Symbol: CLCA2
• Gene ID (NCBI): 9635
• RRID: AB_10642000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IP	Front Cell Dev Biol; Epigenetic Repression of Chloride Channel Accessory 2 Transcription in Cardiac Fibroblast: Implication in Cardiac Fibrosis.; Authors - Tinghui Shao
mouse	WB	Front Cardiovasc Med; Microenvironment Stiffness Amplifies Post-ischemia Heart Regeneration in Response to Exogenous Extracellular Matrix Proteins in Neonatal Mice.; Authors - Xinming Wang
human	IP	Biomedicines; Self-Cleavage of Human Chloride Channel Accessory 2 Causes a Conformational Shift That Depends on Membrane Anchorage and Is Required for Its Regulation of Store-Operated Calcium Entry; Authors - Grace T Ramena
human	WB	J Ovarian Res; TP63 truncating mutation causes increased cell apoptosis and premature ovarian insufficiency by enhanced transcriptional activation of CLCA2; Authors - Yali Fan ; KD Validated

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