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CLCA1 Polyclonal antibody, PBS Only

CLCA1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25291-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLCA1 Polyclonal antibody (25291-1-AP)

    WB analysis using 25291-1-AP (same clone as 25291-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25291-1-AP (CLCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CLCA1 Polyclonal antibody (25291-1-AP)

    IHC staining of human small intestine using 25291-1-AP (same clone as 25291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25291-1-AP (CLCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CLCA1 Polyclonal antibody (25291-1-AP)

    IHC staining of human small intestine using 25291-1-AP (same clone as 25291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25291-1-AP (CLCA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25291-1-PBS in a different storage buffer formulation.

Product Information

25291-1-PBS targets CLCA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLCA1 fusion protein Ag18176
Full Name chloride channel accessory 1
Calculated Molecular Weight 914 aa, 100 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC156805
Gene Symbol CLCA1
Gene ID (NCBI) 1179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Calcium-activated chloride channel regulator 1(CLCA1) is a secreted protein, and it has an N-terminal zinc-dependent metallohydrolase domain with a conserved HExxE catalytic motif similar to that found in matrix metalloproteases (MMPs), and "a Disintegrin and metalloproteases" (ADAMs). The only known substrate for CLCA1 is CLCA1 itself as it undergoes intracellular autocatalytic cleavage resulting in two cleavage products which are both secreted. Since a previous study suggested that a band around 75 kDa, corresponding to the N-terminal autocatalytic cleavage product, was observed in mucus from both human and WT mice. In addition, an additional band at 50 kDa was identified in human and WT mouse mucus, suggesting that CLCA1 can be further processed. However, no band corresponding to the full-length un-cleaved CLCA1 could be detected in the human samples, and only a very faint band was observed in WT mouse mucus, indicating that most of the CLCA1 that is secreted into the mucus is fully cleaved in vivo. (PMID: 29885864)