CKMT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CKMT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 13207-1-AP

Synonyms

Mib-CK, Mib CK, EC:2.7.3.2, Creatine kinase S-type, mitochondrial, CKMT 2

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Validation Data Gallery

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  WB analysis using 13207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13207-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 13207-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

13207-1-AP targets CKMT2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CKMT2 fusion protein Ag3971
• Full Name: creatine kinase, mitochondrial 2 (sarcomeric)
• Calculated Molecular Weight: 419 aa, 48 kDa
• Observed Molecular Weight: 41-48 kDa
• GenBank Accession Number: BC029140
• Gene Symbol: CKMT2
• Gene ID (NCBI): 1160
• RRID: AB_2081188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Metab; The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity.; Authors - Suzhen Chen
chicken	WB	Mol Cell Proteomics; Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens; Authors - Yunlei Li
mouse	WB,IHC	Metabolism; Sustained mitochondrial biogenesis is essential to maintain caloric restriction-induced beige adipocytes.; Authors - Raja Gopal Reddy Mooli
human	WB	Front Cell Dev Biol; Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma; Authors - Wenshuo Zhang
chicken	WB	Mol Cell Proteomics; Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens.; Authors - Yunlei Li
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa