CKMT1A Polyclonal antibody

• Featured Product
• KD/KO Validated

CKMT1A Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 15346-1-AP

Synonyms

Creatine kinase U-type, mitochondrial, CKMT1B, CKMT1, CKMT, CKMiMi

---

Validation Data Gallery

• 

  WB analysis of A549 cells using 15346-1-AP

  A549 cells (shcontrol and shRNA of CKMT1A) were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:30.

• 

  WB analysis of rat heart using 15346-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15346-1-AP

  WB result of CKMT1A antibody (15346-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CKMT1A transfected HEK-293 cells.

• 

  WB analysis of human colon using 15346-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15346-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15346-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15346-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15346-1-AP

  IP result of anti-CKMT1A (IP:15346-1-AP, 4ug; Detection:15346-1-AP 1:1000) with mouse heart tissue lysate 3200ug.

• 

  IHC staining of human colon cancer using 15346-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15346-1-AP (CKMT1A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15346-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15346-1-AP (CKMT1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: rat heart tissue, human colon tissue, human testis tissue, mouse heart tissue, mouse testis tissue, HEK-293 cells, A549 cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• FC: See 1 publications below

Product Information

15346-1-AP targets CKMT1A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CKMT1A fusion protein Ag7583
• Full Name: creatine kinase, mitochondrial 1A
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC001926
• Gene Symbol: CKMT1A
• Gene ID (NCBI): 548596
• RRID: AB_2081073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.

Publications

Species	Application	Title
human	WB	Cell Metab; Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.; Authors - Kiran Kurmi ; KD Validated
human	WB,IHC,IF	Nat Commun; Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation.; Authors - Liang-Jie Wang ; KD Validated
human	IHC, WB	Respir Res; Mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation.; Authors - Ming Li
mouse	IHC	Int J Cancer; Increased serum mitochondrial creatine kinase activity as a risk for hepatocarcinogenesis in chronic hepatitis C patients.; Authors - Kenichiro Enooku
rat	IHC	Front Cell Neurosci; Investigations into photoreceptor energy metabolism during experimental retinal detachment; Authors - Glyn Chidlow
mouse	IF	J Physiol; Mitochondrial phosphagen kinases support the volatile power demands of motor nerve terminals; Authors - Karlis A Justs

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Nusrat (Verified Customer) (08-09-2024)

The antibody yielded a strong, prominent band at about 42 kD and works superbly. It also works well at a 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Porcine HCC cell line