CKM-Specific Monoclonal antibody
CKM-Specific Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2G3F6
Cat no : 60177-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Human skeletal muscle, human skeletal muscle tissue |
| Positive IHC detected in | human heart tissue, mouse heart tissue, mouse testis tissue, rat brain tissue, rat testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | HepG2 cells, C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
60177-1-Ig targets CKM-Specific in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | creatine kinase, muscle |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43 kDa, 90 kDa, 130 kDa |
| GenBank Accession Number | BC007462 |
| Gene Symbol | CKM |
| Gene ID (NCBI) | 1158 |
| RRID | AB_10897180 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium. CKM has a calculated molecular mass of 43 kDa, and the 90-kDa and 130-kDa bands could be due to acovalent cross-linking of two and three CKm subunits, respectively (PMID: 20195383).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CKM-Specific antibody 60177-1-Ig | Download protocol |
| IHC protocol for CKM-Specific antibody 60177-1-Ig | Download protocol |
| IF protocol for CKM-Specific antibody 60177-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Novel small molecule DMAMCL induces differentiation in rhabdomyosarcoma by downregulating of DLL1 |
