CKM-Specific Polyclonal antibody

CKM-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18712-1-AP

Synonyms

CKMM, Creatine Kinase MM, Creatine Kinase M-Specific, Creatine kinase M chain, Creatine kinase M type, creatine kinase, muscle, M CK

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Validation Data Gallery

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  WB analysis using 18712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18712-1-AP (CKM-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18712-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18712-1-AP (CKM-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 18712-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18712-1-AP (CKM-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18712-1-AP (CKM-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18712-1-AP (CKM-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart using 18712-1-AP (CKM-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat heart using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart using 18712-1-AP (CKM-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, mouse small intestine tissue, mouse sketetal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue, rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

18712-1-AP targets CKM-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, Bacterial
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: creatine kinase, muscle
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC007462
• Gene Symbol: CKM
• Gene ID (NCBI): 1158
• RRID: AB_2260650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42 kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody is specific to CKM.

Publications

Species	Application	Title
	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
mouse	WB	Cell Prolif; KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.; Authors - Jing Hao
mouse	WB	Toxicology; Pb inhibited C2C12 myoblast differentiation by regulating HDAC2; Authors - Xiaozhen Gu
Bacterial	WB	Mol Pharmacol; Naturally Occurring Mutations to Muscle-Type Creatine Kinase Impact Its Canonical and Pharmacological Activities in a Substrate-Dependent Manner In Vitro.; Authors - Eric P Mosher
mouse	WB	BMC Musculoskelet Disord; Ang-(1-7) protects skeletal muscle function in aged mice.; Authors - Ying Li