CKM/CKB Polyclonal antibody, PBS Only

CKM/CKB Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15891-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse heart using 15891-1-AP (same clone as 15891-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 15891-1-AP (same clone as 15891-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 15891-1-AP (same clone as 15891-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15891-1-AP (same clone as 15891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 15891-1-AP (same clone as 15891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

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Product Information

15891-1-PBS targets CKM/CKB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CKM/CKB fusion protein Ag8678
• Full Name: creatine kinase, muscle
• Calculated Molecular Weight: 381 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC007462
• Gene Symbol: CKM
• Gene ID (NCBI): 1158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes(muscle, brain, sarcomeric, Ubiquitous-type) play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody can recognize both CKB and CKM due to the high homology.