CKB-Specific Polyclonal antibody

CKB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18713-1-AP

Synonyms

CKB, KCRB, Creatine kinase B-type, CPK-B, CKBB

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Validation Data Gallery

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  WB analysis of HEK-293 using 18713-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 18713-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 18713-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18713-1-AP (CKB-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18713-1-AP (CKB-Specific Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 18713-1-AP (CKB-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 18713-1-AP (CKB-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HEK-293 using 18713-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 18713-1-AP (CKB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse colon tissue, Y79 cells
• Positive IHC detected in: mouse brain tissue, human ovary tumor tissue, human gliomas tissue, mouse skeletal muscle tissue, mouse testis tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18713-1-AP targets CKB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: creatine kinase, brain
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC001190
• Gene Symbol: CKB
• Gene ID (NCBI): 1152
• RRID: AB_10596470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody is specific to CKB.