CKAP2L Polyclonal antibody

• Featured Product
• KD/KO Validated

CKAP2L Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17143-1-AP

Synonyms

Radmis, Radial fiber and mitotic spindle protein, Cytoskeleton-associated protein 2-like

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Validation Data Gallery

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  WB analysis using 17143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17143-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17143-1-AP

  IP result of anti-CKAP2L (IP:17143-1-AP, 4ug; Detection:17143-1-AP 1:2000) with Jurkat cells lysate 1560 ug.

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  IHC staining of human colon cancer using 17143-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

17143-1-AP targets CKAP2L in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CKAP2L fusion protein Ag10909
• Full Name: cytoskeleton associated protein 2-like
• Calculated Molecular Weight: 745 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC036217
• Gene Symbol: CKAP2L
• Gene ID (NCBI): 150468
• RRID: AB_2080720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Int J Clin Exp Pathol; Oncogenic and prognostic role of CKAP2L in hepatocellular carcinoma.; Authors - Penghui Wang ; KD Validated
human	WB,IHC	FASEB J; IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m6 A-dependent manner; Authors - Yaqian Shi