CISD1 Monoclonal antibody

CISD1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D7E3

Cat no : 68030-1-Ig

Synonyms

EC:2.6.1.3, Cysteine transaminase CISD1, CDGSH iron-sulfur domain-containing protein 1, CDGSH iron sulfur domain 1, C10orf70

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Validation Data Gallery

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  WB analysis using 68030-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68030-1-Ig (CISD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 68030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68030-1-Ig (CISD1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 68030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68030-1-Ig (CISD1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of H9C2 using 68030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using CISD1 antibody (68030-1-Ig, Clone: 1D7E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, pig brain tissue, rabbit brain tissue, rat brian tissue, mouse brain tissue, chicken brain tissue, mouse cerebellum tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: H9C2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68030-1-Ig targets CISD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CISD1 fusion protein Ag8560
• Full Name: CDGSH iron sulfur domain 1
• Calculated Molecular Weight: 108 aa, 12 kDa
• Observed Molecular Weight: 14-17 kDa
• GenBank Accession Number: BC007043
• Gene Symbol: CISD1
• Gene ID (NCBI): 55847
• RRID: AB_2918772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.