Cirhin Polyclonal antibody

Cirhin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11057-1-AP

Synonyms

CIRH1A, Cirhin, KIAA1988, NAIC, TEX292, UTP4

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Validation Data Gallery

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  WB analysis using 11057-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11057-1-AP (Cirhin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11057-1-AP

  IP result of anti-Cirhin (IP:11057-1-AP, 4ug; Detection:11057-1-AP 1:700) with HepG2 cells lysate 2600 ug.

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  IHC staining of mouse brain using 11057-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11057-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Neuro-2a cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11057-1-AP targets Cirhin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cirhin fusion protein Ag1494
• Full Name: cirrhosis, autosomal recessive 1A (cirhin)
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 70-77 kDa
• GenBank Accession Number: BC009348
• Gene Symbol: Cirhin
• Gene ID (NCBI): 84916
• RRID: AB_2877743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cirhin is a protein that in humans is encoded by the CIRH1A gene. Mutation of this gene has been associated with North American Indian childhood cirrhosis, a severe intrahepatic cholestasis that results in transient neonatal jaundice, and progresses to periportal fibrosis and cirrhosis in childhood and adolescence (PMID: 12417987). Catalog#11057-1-AP is a rabbit polyclonal antibody raised against the full-length of human CIRHIN.