CIP29 Polyclonal antibody

CIP29 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15798-1-AP

Synonyms

Proliferation-associated cytokine-inducible protein CIP29, Nuclear protein Hcc-1, HSPC316, HCC1, Cytokine-induced protein of 29 kDa

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Validation Data Gallery

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  WB analysis using 15798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15798-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15798-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 15798-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15798-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15798-1-AP (CIP29 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15798-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 15798-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Raji cells, rat pancreas tissue, HeLa cells, MCF-7 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

15798-1-AP targets CIP29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIP29 fusion protein Ag8507
• Full Name: cytokine induced protein 29 kDa
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 29-32 kDa
• GenBank Accession Number: BC007099
• Gene Symbol: CIP29
• Gene ID (NCBI): 84324
• RRID: AB_1959356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Tumour Biol; Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.; Authors - Ying Zhu
human	WB,IF	PLoS Pathog; TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus; Authors - Lingcai Zhao ; KD Validated