CIN85 Polyclonal antibody

• Featured Product
• KD/KO Validated

CIN85 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12132-1-AP

Synonyms

SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3

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Validation Data Gallery

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  WB analysis using 12132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 12132-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 12132-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12132-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12132-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Raji cells, fetal human brain tissue, Ramos cells, Jurkat cells, THP-1 cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12132-1-AP targets CIN85 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIN85 fusion protein Ag2777
• Full Name: SH3-domain kinase binding protein 1
• Calculated Molecular Weight: 665 aa, 73 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC015806
• Gene Symbol: SH3KBP1/CIN85
• Gene ID (NCBI): 30011
• RRID: AB_2187277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

Publications

Species	Application	Title
human	WB, IF	J Cell Biol; CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface.; Authors - Ihor Yakymovych
human	WB	Biol Cell; ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.; Authors - Yoon Hye-Young HY ; KD Validated
human	IHC	Arch Gynecol Obstet; Significance of PTPRZ1 and CIN85 expression in cervical carcinoma.; Authors - Ma Yaxi Y