CHST3 Polyclonal antibody

CHST3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 18242-1-AP

Synonyms

C6ST, C6ST 1, C6ST1, Chondroitin 6 sulfotransferase, CHST3, GST 0, HSD

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Validation Data Gallery

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  WB analysis of mouse heart using 18242-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18242-1-AP (CHST3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18242-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18242-1-AP (CHST3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18242-1-AP (CHST3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18242-1-AP (CHST3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 18242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18242-1-AP (CHST3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human breast cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

18242-1-AP targets CHST3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHST3 fusion protein Ag13008
• Full Name: carbohydrate (chondroitin 6) sulfotransferase 3
• Calculated Molecular Weight: 479 aa, 55 kDa
• Observed Molecular Weight: 55-67 kDa
• GenBank Accession Number: BC093690
• Gene Symbol: CHST3
• Gene ID (NCBI): 9469
• RRID: AB_10694672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion.; Authors - Laura Hobohm
human	IHC	Osteoarthritis Cartilage; Altered expression of chondroitin sulfate structure modifying sulfotransferases in the articular cartilage from adult osteoarthritis and Kashin-Beck disease.; Authors - Jing Han
mouse	IF	Cells; Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3.; Authors - David Baidoe-Ansah
mouse	IHC	J Neurochem; OASIS regulates chondroitin 6-O-sulfotransferase 1 gene transcription in the injured adult mouse cerebral cortex.; Authors - Hiroaki Okuda
human	IHC	Histochem Cell Biol; Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease.; Authors - Jian Lei
human	WB	Biol Trace Elem Res; Serious Selenium Deficiency in the Serum of Patients with Kashin-Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes.; Authors - Liyun Wang