CHST14 Polyclonal antibody

CHST14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17749-1-AP

Synonyms

CHST14, D4ST 1, D4ST1, Dermatan 4 sulfotransferase 1, HD4ST, hD4ST1, HNK1ST

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Validation Data Gallery

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  WB analysis of HEK-293 using 17749-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17749-1-AP (CHST14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17749-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17749-1-AP (CHST14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 17749-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 17749-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 17749-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 17749-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17749-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 17749-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17749-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17749-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IHC detected in: human placenta tissue, human brain tissue, human kidney tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17749-1-AP targets CHST14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHST14 fusion protein Ag12014
• Full Name: carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14
• Calculated Molecular Weight: 376 aa, 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC049214
• Gene Symbol: CHST14
• Gene ID (NCBI): 113189
• RRID: AB_2079051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Transl Oncol; A comprehensive analysis of the expression, immune infiltration, prognosis and partial experimental validation of CHST family genes in gastric cancer; Authors - Jinbo Zhan