CHRNG Polyclonal antibody

CHRNG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21928-1-AP

Synonyms

ACHRG, Acetylcholine receptor subunit gamma

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Validation Data Gallery

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  WB analysis using 21928-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21928-1-AP (CHRNG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21928-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21928-1-AP (CHRNG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21928-1-AP targets CHRNG in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHRNG fusion protein Ag16505
• Full Name: cholinergic receptor, nicotinic, gamma
• Calculated Molecular Weight: 465 aa, 52 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC111802
• Gene Symbol: CHRNG
• Gene ID (NCBI): 1146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetylcholine receptor subunit gamma is a protein that in humans is encoded by the CHRNG gene. The mammalian muscle-type acetylcholine receptor is a transmembrane pentameric glycoprotein with two alpha subunits, one beta, one delta, and one epsilon (in adult skeletal muscle) or gamma (in fetal and denervated muscle) subunit. This gene, which encodes the gamma subunit, is expressed prior to the thirty-third week of gestation in humans. The gamma subunit of the acetylcholine receptor plays a role in neuromuscular organogenesis and ligand binding and disruption of gamma subunit expression prevents the correct localization of the receptor in cell membranes. Mutations in this gene cause Escobar syndrome and a lethal form of multiple pterygium syndrome. Muscle-type acetylcholine receptor is the major antigen in the autoimmune disease myasthenia gravis.