CHRNB2 Polyclonal antibody

CHRNB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17844-1-AP

Synonyms

CHRNB2, EFNL3, nAChRB2



Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 17844-1-AP is CHRNB2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNB2 fusion protein Ag12208
Full Name cholinergic receptor, nicotinic, beta 2 (neuronal)
Calculated Molecular Weight 502 aa, 57 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC075041
Gene Symbol CHRNB2
Gene ID (NCBI) 1141
RRIDAB_2878450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHRNB2 antibody 17844-1-APDownload protocol
IHC protocol for CHRNB2 antibody 17844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum

Authors - Ya-Juan Wang