CHRNA7 Recombinant antibody, PBS Only

CHRNA7 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240117D12

Cat no : 82848-4-PBS

Synonyms

Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 240117D12

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Validation Data Gallery

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  WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 82848-4-RR (same clone as 82848-4-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Chrna7 Recombinant Antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 82848-4-RR (same clone as 82848-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CHRNA7 Recombinant antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82848-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82848-4-RR against Human CHRNA7 were performed. The affinity constant is 4.96 nM.

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Product Information

The immunogen of 82848-4-PBS is CHRNA7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CHRNA7 fusion protein Ag15980
• Full Name: cholinergic receptor, nicotinic, alpha 7
• Calculated Molecular Weight: 502 aa, 56 kDa
• Observed Molecular Weight: 50 kDa, 56 kDa
• GenBank Accession Number: BC037571
• Gene Symbol: CHRNA7
• Gene ID (NCBI): 1139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)