CHRNA7 (48 kDa) Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

CHRNA7 (48 kDa) Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A10

Cat no : 82848-3-PBS

Synonyms

CHRNA7, Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 2A10

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Validation Data Gallery

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  WB analysis using 82848-3-RR (same clone as 82848-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82848-3-RR (CHRNA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 82848-3-RR (same clone as 82848-3-PBS)

  WB result of CHRNA7 antibody (82848-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7 transfected Jurkat cells. This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat dorsal root ganglion using 82848-3-RR (same clone as 82848-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat dorsal root ganglion using 82848-3-RR (same clone as 82848-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82848-3-RR (same clone as 82848-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82848-3-RR (same clone as 82848-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82848-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82848-3-RR against Human CHRNA7 (48 kDa) were performed. The affinity constant is 2.04 nM.

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Product Information

82848-3-PBS targets CHRNA7 (48 kDa) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CHRNA7 (48 kDa) fusion protein Ag15980
• Full Name: cholinergic receptor, nicotinic, alpha 7
• Calculated Molecular Weight: 502 aa, 56 kDa
• Observed Molecular Weight: ~50 kDa
• GenBank Accession Number: BC037571
• Gene Symbol: CHRNA7
• Gene ID (NCBI): 1139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)