CHRNA7 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHRNA7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 21379-1-AP

Synonyms

Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2

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Validation Data Gallery

• 

  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21379-1-AP

  WB result of CHRNA7 antibody (21379-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7 transfected Jurkat cells.

• 

  WB analysis of rat brain using 21379-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21379-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21379-1-AP (CHRNA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21379-1-AP (CHRNA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21379-1-AP (CHRNA7 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21379-1-AP (CHRNA7 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, rat brain tissue, mouse skeletal muscle tissue, HL-60 cells, MCF-7 cells, SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• FC: See 1 publications below

Product Information

21379-1-AP targets CHRNA7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHRNA7 fusion protein Ag15980
• Full Name: cholinergic receptor, nicotinic, alpha 7
• Calculated Molecular Weight: 502 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC037571
• Gene Symbol: CHRNA7
• Gene ID (NCBI): 1139
• RRID: AB_2878852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

Publications

Species	Application	Title
mouse	WB	J Neuroinflammation; Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders; Authors - Jingxian Lun
mouse	WB,IHC	Biomed Pharmacother; Sinomenine regulates the cholinergic anti-inflammatory pathway to inhibit TLR4/NF-κB pathway and protect the homeostasis in brain and gut in scopolamine-induced Alzheimer's disease mice; Authors - Haojie Ni
human	WB	J Invest Dermatol; Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.; Authors - Jing Wang
mouse	WB,IF	Front Pharmacol; Autophagy is Involved in Neuroprotective Effect of Alpha7 Nicotinic Acetylcholine Receptor on Ischemic Stroke.; Authors - Zhe-Qi Xu
rat	WB,IF	Front Pharmacol; Activation of α7nAChR Protects Against Gastric Inflammation and Dysmotility in Parkinson's Disease Rats.; Authors - Li Zhou
mouse	WB	J Alzheimers Dis; Gx-50 Inhibits Neuroinflammation via α7 nAChR Activation of the JAK2/STAT3 and PI3K/AKT Pathways; Authors - Shi Shi

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