CHRNA5 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CHRNA5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G8C11

Cat no : 66363-1-PBS

Synonyms

CHRNA5, LNCR2, NACHRA5

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 66363-1-Ig (same clone as 66363-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig skeletal muscle using 66363-1-Ig (same clone as 66363-1-PBS)

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66363-1-Ig (same clone as 66363-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C2C12 using 66363-1-Ig (same clone as 66363-1-PBS)

  C2C12 cell were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 66363-1-Ig (same clone as 66363-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bladder using 66363-1-Ig (same clone as 66363-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bladder using 66363-1-Ig (same clone as 66363-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 66363-1-Ig (same clone as 66363-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 66363-1-Ig (same clone as 66363-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66363-1-PBS targets CHRNA5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHRNA5 fusion protein Ag4447
• Full Name: cholinergic receptor, nicotinic, alpha 5
• Calculated Molecular Weight: 468 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC033639
• Gene Symbol: CHRNA5
• Gene ID (NCBI): 1138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.