CHRNA5 Polyclonal antibody, PBS Only

CHRNA5 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13516-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of Jurkat using 13516-1-AP (same clone as 13516-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13516-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 13516-1-AP (same clone as 13516-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13516-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse stomach using 13516-1-AP (same clone as 13516-1-PBS)

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13516-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse bladder using 13516-1-AP (same clone as 13516-1-PBS)

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13516-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 13516-1-AP (same clone as 13516-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13516-1-PBS in a different storage buffer formulation.

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Product Information

13516-1-PBS targets CHRNA5 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHRNA5 fusion protein Ag4447
• Full Name: cholinergic receptor, nicotinic, alpha 5
• Calculated Molecular Weight: 468 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC033639
• Gene Symbol: CHRNA5
• Gene ID (NCBI): 1138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.