CHRNA3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CHRNA3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10333-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 10333-1-AP (same clone as 10333-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 10333-1-AP (same clone as 10333-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10333-1-AP (same clone as 10333-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10333-1-AP (same clone as 10333-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10333-1-AP (same clone as 10333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10333-1-AP (CHRNA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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Product Information

10333-1-PBS targets CHRNA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHRNA3 fusion protein Ag0272
• Full Name: cholinergic receptor, nicotinic, alpha 3
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000513
• Gene Symbol: CHRNA3
• Gene ID (NCBI): 1136
• RRID: AB_2080511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CHRNA3, also named as NACHRA3, LNCR2 and PAOD2, is neuronal type nAChR subunits. It is a member of the nicotinic acetylcholine receptor family, which plays an important role in calcium regulation, neuronal development, and cognitive functions. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer.