CHRNA7/CHRFAM7A Polyclonal antibody

• Featured Product
• KD/KO Validated

CHRNA7/CHRFAM7A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21586-1-AP

Synonyms

CHRFAM7A, CHRNA7, CHRNA7 DR1, CHRNA7 FAM7A fusion protein, CHRNA7/CHRFAM7A, D 10

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Validation Data Gallery

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  WB analysis of Jurkat using 21586-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21586-1-AP (CHRNA7/CHRFAM7A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21586-1-AP

  WB result of CHRNA7/CHRFAM7A antibody (21586-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7/CHRFAM7A transfected Jurkat cells.

• 

  WB analysis of Jurkat using 21586-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21586-1-AP (CHRNA7/CHRFAM7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21586-1-AP targets CHRNA7/CHRFAM7A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHRNA7/CHRFAM7A fusion protein Ag16151
• Full Name: CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion
• Calculated Molecular Weight: 412 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC101348
• Gene Symbol: CHRNA7/CHRFAM7A
• Gene ID (NCBI): 89832
• RRID: AB_10734326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuronal acetylcholine receptor subunit alpha-7, encoded by the CHRNA7 gene, is involved in cognition through interneuron modulation of 4-(2-aminoethyl)benzene-1,2-diol and glutamate signaling. CHRNA7 gene is genetically linked to multiple disorders with cognitive deficits. A partial duplication of CHRNA7 (CHRFAM7A) is found in humans. CHRFAM7A gene encodes the CHRNA7-FAM7A fusion protein. Expression of CHRFAM7A has been reported in brain, peripheral blood lymphocytes (PBLs), and a broad range of epithelial cells (PMID: 21718690; 23553139; 25681457). CHRFAM7A has been associated with many neuropsychiatric disorders (PMID: 25906356; 25701707). This antibody, raised against 241-386 amino acids of human CHRFAM7A protein, recognizes both CHRNA7 and CHRFAM7A.