CHMP7 Monoclonal antibody

CHMP7 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8F6

Cat no : 68406-1-Ig

Synonyms

CHMP family, member 7, CHMP7, Chromatin modifying protein 7

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Validation Data Gallery

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  WB analysis using 68406-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68406-1-Ig (CHMP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IHC staining of human prostate cancer using 68406-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68406-1-Ig (CHMP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 68406-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68406-1-Ig (CHMP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 68406-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (68406-1-Ig, Clone: 2F8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68406-1-Ig targets CHMP7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHMP7 fusion protein Ag9641
• Full Name: CHMP family, member 7
• Calculated Molecular Weight: 453 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC019110
• Gene Symbol: CHMP7
• Gene ID (NCBI): 91782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.