CHMP4B Recombinant antibody, PBS Only

CHMP4B Uni-rAb^TM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2N22

Cat no : 81691-2-PBS

Synonyms

hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B, C20orf178

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Validation Data Gallery

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  WB analysis using 81691-2-RR (same clone as 81691-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81691-2-RR (CHMP4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81691-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 81691-2-RR (same clone as 81691-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CHMP4B Recombinant antibody (81691-2-RR, Clone:2N22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81691-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81691-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81691-2-RR against Human CHMP4B were performed. The affinity constant is 4.59 nM.

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Product Information

The immunogen of 81691-2-PBS is CHMP4B Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CHMP4B fusion protein Ag4544
• Full Name: chromatin modifying protein 4B
• Calculated Molecular Weight: 224 aa, 25 kDa
• GenBank Accession Number: BC033859
• Gene Symbol: CHMP4B
• Gene ID (NCBI): 128866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.