CHL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25250-1-AP

Synonyms

CALL, CHL1, Close homolog of L1, L1CAM2

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Validation Data Gallery

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  WB analysis of mouse brain using 25250-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25250-1-AP (CHL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 25250-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 25250-1-AP (CHL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, COS-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

The immunogen of 25250-1-AP is CHL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHL1 fusion protein Ag19263
• Full Name: cell adhesion molecule with homology to L1CAM (close homolog of L1)
• Calculated Molecular Weight: 1208 aa, 135 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC104918
• Gene Symbol: CHL1
• Gene ID (NCBI): 10752
• RRID: AB_2879988
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Sci Transl Med; Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans.; Authors - Sabrina Simoes
human	WB	Oncol Lett; Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway.; Authors - Xiangdao Cai ; KD Validated
human	WB	Exp Ther Med; CHL1 inhibits cell proliferation, migration and invasion by regulating the NF‑κB signaling pathway in colorectal cancer; Authors - Ming Bao