Chk1 Polyclonal antibody

Chk1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 25887-1-AP

Synonyms

CHEK1, CHK1



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293T cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25887-1-AP is Chk1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chk1 fusion protein Ag22993
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated molecular weight 54 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC004202
Gene symbol CHEK1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for Chk1 antibody 25887-1-APDownload protocol
IHC protocol for Chk1 antibody 25887-1-APDownload protocol
IF protocol for Chk1 antibody 25887-1-APDownload protocol
IP protocol for Chk1 antibody 25887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Exp Clin Cancer Res

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Authors - Jingyuan Sun
  • KD Validated
humanWB

Cell Death Discov

Inhibition of STAT3Y705 phosphorylation by Stattic suppresses proliferation and induces mitochondrial-dependent apoptosis in pancreatic cancer cells.

Authors - Hangcheng Guo
humanWB

Biochem Biophys Res Commun

ACTL6A deficiency induces apoptosis through impairing DNA replication and inhibiting the ATR-Chk1 signaling in glioblastoma cells.

Authors - Xiaosong Hu
humanWB

Med Oncol

PMID: 31372848

Authors - Jingrui Jin
humanWB

Front Pharmacol

Induction of Mitosis Delay and Apoptosis by CDDO-TFEA in Glioblastoma Multiforme.

Authors - Tai-Hsin Tsai
humanWB

EBioMedicine

MAP4K1 functions as a tumor promotor and drug mediator for AML via modulation of DNA damage/repair system and MAPK pathway.

Authors - Qing Ling