Chromogranin A Polyclonal antibody

Chromogranin A Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23342-1-AP

Synonyms

CHGA, CGA, ER-37, EA-92, Chromogranin-A

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Validation Data Gallery

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  IHC staining of human lung cancer using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human small intestine using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human appendicitis using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human colon using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of mouse colon using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23342-1-AP (Chromogranin A antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of mouse colon using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23342-1-AP (Chromogranin A antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human lung cancer using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 23342-1-AP (Chromogranin A antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 23342-1-AP (Chromogranin A antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse small intestine using 23342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (23342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 23342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (23342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 23342-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Chromogranin A antibody (23342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse pancreas tissue, human lung cancer tissue, human small intestine tissue, human colon tissue, mouse colon tissue, human appendicitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse small intestine tissue
• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

23342-1-AP targets Chromogranin A in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Chromogranin A fusion protein Ag19780
• Full Name: chromogranin A (parathyroid secretory protein 1)
• Calculated Molecular Weight: 51 kDa
• GenBank Accession Number: BC006459
• Gene Symbol: Chromogranin A
• Gene ID (NCBI): 1113
• RRID: AB_2879259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Publications

Species	Application	Title
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
mouse	IF	Cell Mol Gastroenterol Hepatol; Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia; Authors - Pawan Puri