CHEK2 Polyclonal antibody

CHEK2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13954-1-AP


CDS1, CHEK2, Chk2, HuCds1, LFS2, PP1425, RAD53

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells
Positive IP detected inHL-60 cells
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 17 publications below

Product Information

13954-1-AP targets CHEK2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHEK2 fusion protein Ag5065
Full Name CHK2 checkpoint homolog (S. pombe)
Calculated molecular weight 61 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC004207
Gene symbol CHEK2
Gene ID (NCBI) 11200
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for CHEK2 antibody 13954-1-APDownload protocol
IF protocol for CHEK2 antibody 13954-1-APDownload protocol
IP protocol for CHEK2 antibody 13954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Authors - Jieyuan Wu

Int J Nanomedicine

TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.

Authors - Bin Han

Cell Death Discov

Comparable radiation sensitivity in p53 wild-type and p53 deficient tumor cells associated with different cell death modalities.

Authors - Ping Li

Anticancer Res

Azidothymidine (AZT) Inhibits Proliferation of Human Ovarian Cancer Cells by Regulating Cell Cycle Progression.

Authors - Yeunting Hsieh

J Sci Food Agric

Rice protein depresses DNA damage through activating p53 pathway and stimulating endogenous antioxidant response in growing and adult rats.

Authors - Mingcai Liang

Plant Foods Hum Nutr

Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.

Authors - Jie Wei