CHEK2 Polyclonal antibody

CHEK2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 13954-1-AP

Synonyms

CDS1, CHEK2, Chk2, HuCds1, LFS2, PP1425, RAD53

---

Validation Data Gallery

• 

  WB analysis of HL-60 using 13954-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13954-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13954-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 13954-1-AP

  IP Result of anti-Chk2 (IP:13954-1-AP, 4ug; Detection:13954-1-AP 1:500) with HL-60 cells lysate 1600ug.

• 

  IF Staining of HepG2 using 13954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHEK2 antibody (13954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phallodin (red).

---

Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells
• Positive IP detected in: HL-60 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 17 publications below

Product Information

13954-1-AP targets CHEK2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHEK2 fusion protein Ag5065
• Full Name: CHK2 checkpoint homolog (S. pombe)
• Calculated molecular weight: 61 kDa
• Observed molecular weight: 65 kDa
• GenBank accession number: BC004207
• Gene symbol: CHEK2
• Gene ID (NCBI): 11200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Biometals; Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.; Authors - Jieyuan Wu
rat	WB	Int J Nanomedicine; TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.; Authors - Bin Han
human	WB	Cell Death Discov; Comparable radiation sensitivity in p53 wild-type and p53 deficient tumor cells associated with different cell death modalities.; Authors - Ping Li
human	WB	Anticancer Res; Azidothymidine (AZT) Inhibits Proliferation of Human Ovarian Cancer Cells by Regulating Cell Cycle Progression.; Authors - Yeunting Hsieh
rat	WB	J Sci Food Agric; Rice protein depresses DNA damage through activating p53 pathway and stimulating endogenous antioxidant response in growing and adult rats.; Authors - Mingcai Liang
mouse	WB	Plant Foods Hum Nutr; Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.; Authors - Jie Wei