CHEK2 Polyclonal antibody
CHEK2 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, ELISA
Conjugate
Unconjugated
Cat no : 13954-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, HeLa cells |
Positive IP detected in | HL-60 cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 17 publications below |
Product Information
13954-1-AP targets CHEK2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CHEK2 fusion protein Ag5065 |
Full Name | CHK2 checkpoint homolog (S. pombe) |
Calculated molecular weight | 61 kDa |
Observed molecular weight | 65 kDa |
GenBank accession number | BC004207 |
Gene symbol | CHEK2 |
Gene ID (NCBI) | 11200 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for CHEK2 antibody 13954-1-AP | Download protocol |
IF protocol for CHEK2 antibody 13954-1-AP | Download protocol |
IP protocol for CHEK2 antibody 13954-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biometals Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex. | ||
Int J Nanomedicine TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats. | ||
Cell Death Discov Comparable radiation sensitivity in p53 wild-type and p53 deficient tumor cells associated with different cell death modalities. | ||
Anticancer Res Azidothymidine (AZT) Inhibits Proliferation of Human Ovarian Cancer Cells by Regulating Cell Cycle Progression. | ||
J Sci Food Agric Rice protein depresses DNA damage through activating p53 pathway and stimulating endogenous antioxidant response in growing and adult rats. | ||
Plant Foods Hum Nutr Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver. |