CHEK2 Polyclonal antibody

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  WB analysis of HL-60 using 13954-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13954-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13954-1-AP

  WB result of CHEK2 antibody (13954-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHEK2 transfected HepG2 cells.

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  WB analysis of HL-60 using 13954-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13954-1-AP

  IP result of anti-CHEK2 (IP:13954-1-AP, 4ug; Detection:13954-1-AP 1:4000) with HeLa cells lysate 1320 ug.

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  IF Staining of HepG2 using 13954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHEK2 antibody (13954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phallodin (red).

• Featured Product
• KD/KO Validated

CHEK2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 13954-1-AP

Publications(37)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CHK2, Hucds1, hCds1, EC:2.7.11.1, Cds1 homolog

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 35 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13954-1-AP targets CHEK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHEK2 fusion protein Ag5065
• Full Name: CHK2 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC004207
• Gene Symbol: CHEK2
• Gene ID (NCBI): 11200
• RRID: AB_10598159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O96017
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Death Differ; ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity; Authors - Feng Xu
human	WB	Nat Commun; G-quadruplex DNA structures in human stem cells and differentiation.; Authors - Katherine G Zyner
		PLoS Biol; A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancers.; Authors - Kunming Zhao
human	WB	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
human	WB	Clin Transl Med; Single-cell profiling-guided combination therapy of c-Fos and histone deacetylase inhibitors in diffuse large B-cell lymphoma.; Authors - Luqiao Wang
rat	WB	Int J Nanomedicine; TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.; Authors - Bin Han

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (01-17-2023)

I have used this antibody for human keratinocytes, cardiomyocytes, mouse skin and liver tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin