CHD4 Monoclonal antibody, PBS Only

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  WB analysis of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

  WB result of CHD4 antibody (66222-1-Ig, 1:1000) with si-Control and si-CHD4 transfected HEK293 cells. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66222-1-Ig (same clone as 66222-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66222-1-Ig (same clone as 66222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66222-1-Ig (CHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66222-1-Ig (same clone as 66222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66222-1-Ig (CHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HEK-293 cells using 66222-1-Ig(CHD4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CHD4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66222-1-PBS

Clone No.3D9G2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA

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Product Information

66222-1-PBS targets CHD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHD4 fusion protein Ag5729
• Full Name: chromodomain helicase DNA binding protein 4
• Calculated Molecular Weight: 1937 aa, 221 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: BC038596
• Gene Symbol: CHD4
• Gene ID (NCBI): 1108
• RRID: AB_2881613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q14839
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.