CHD4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (2)

Cat no : 14173-1-AP

Synonyms

Mi 2b, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 4, CHD-4, CHD 4

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Validation Data Gallery

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  WB analysis of HEK-293 using 14173-1-AP

  WB result of CHD4 antibody (14173-1-AP, 1:1000) with si-Control and si-CHD4 transfected HEK293 cells.

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  WB analysis using 14173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14173-1-AP (CHD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14173-1-AP (CHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14173-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14173-1-AP (CHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14173-1-AP

  IP result of anti-CHD4 (IP:14173-1-AP, 4ug; Detection:14173-1-AP 1:300) with HeLa cells lysate 2640 ug.

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  IP experiment of HeLa using 14173-1-AP

  IP result of anti-CHD4 (IP:14173-1-AP, 4ug; Detection:14173-1-AP 1:1000) with HeLa cells lysate 1880 ug.

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  IHC staining of human cervical cancer using 14173-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14173-1-AP (CHD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14173-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14173-1-AP (CHD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14173-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD4 antibody (14173-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A549 using 14173-1-AP

  Immunofluorescent analysis of A549 cells, using CHD4 antibody 14173-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Product Information

14173-1-AP targets CHD4 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHD4 fusion protein Ag5372
• Full Name: chromodomain helicase DNA binding protein 4
• Calculated Molecular Weight: 1937 aa, 221 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: BC038596
• Gene Symbol: CHD4
• Gene ID (NCBI): 1108
• RRID: AB_2080046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calculated molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.; Authors - Xianjiang Lan
		Leukemia; Runx1 repression by histone deacetylation is critical for Setbp1-induced mouse myeloid leukemia development.; Authors - B A Vishwakarma
human	ChIP,CoIP	Cancer Res; CHD4 Promotes Breast Cancer Progression as a Coactivator of Hypoxia-Inducible Factors.; Authors - Yijie Wang ; KD Validated
human	WB,ChIP	Arthritis Rheumatol; Functional Genomic Analysis of a RUNX3 Polymorphism Associated with Ankylosing Spondylitis.; Authors - Matteo Vecellio
human	WB,IF	Clin Epigenetics; CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma.; Authors - Simin Shao ; KD Validated
human	WB,IHC	Cell Signal; CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer; Authors - Jun Zhang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaochen (Verified Customer) (07-31-2023)

It is very sensitive and accurate. I can get the sepecific bend easily.

• Applications: Western Blot

CHD4 Antibody Western Blot validation ( dilution) in (Cat no:14173-1-AP)

FH

Christos (Verified Customer) (01-26-2023)

Application: IF Sample: Human Cell line (HeLa Kyoto) Fixation: Paraformaldehyde (PFA 4% in PHEM pH=6.9) at room temperature for 15 minutes Permeabilization: 0.1% Triton-X-100 in PBS at room temperature for 5 minutes Blocking step: BSA as blocking agent for 1 hour · Concentration: 5% BSA in PBS · Temperature: RT dilution of the primary antibody: 1/50 incubation time: overnight at 4°C DNA (blue), CHD4 (green) and α-tubulin (red)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Human cervix carcinoma cells/ HeLe Kyoto

CHD4 Antibody Immunofluorescence validation (1/50 dilution) in Human cervix carcinoma cells/ HeLe Kyoto (Cat no:14173-1-AP)